Discovery Science Research Project
Cruciferous Complete
Kale growing in test beds

The research and development department is currently researching the ability of cruciferous vegetables such as Brussels sprouts and kale increase detoxification enzymes. The in vitro model that we use consists of human liver cells that we treat with extracts from cruciferous vegetables that we collect from the farm, or extracts that we get from the products themselves. Using an enzyme activity assay and Real-Time PCR to measure the RNA levels of the detoxification enzymes, we have demonstrated that kale, Brussels sprouts, and Cruciferous Complete all increase detoxification enzyme levels in our in vitro model using human liver cells. This research assures that Standard Process products are made from high quality ingredients that retain expected biological activity throughout our manufacturing process.

mRNA Expression


Extracts from kale, Brussels sprouts, and Cruciferous Complete increase Phase I detoxification enzyme RNA levels. HepG2 cells were treated for 2 days with the extract from 5 mg of kale and Brussels sprouts powder made at our facility, or Cruciferous Complete. Real-time PCR was used to determine that the extracts from all three materials increased the expression of mRNA for the Phase I detoxification enzymes cytochrome P450 CYP1A1, CYP1A2, and CYP3A4 in HepG2 cells.
 
Quinone Reduction Activity


Extracts from kale, Brussels sprouts, and Cruciferous Complete increase quinone reductase activity in HepG2 human liver cells.HepG2 cells were treated for 2 days with the extract from 1, 5 and 10 mg of kale and Brussels sprouts powder made in our facility, or Cruciferous Complete. An enzymatic activity assay was used to determine that at concentrations of 5 and 10 mg, the extracts from all three materials increased the activity of quinone reductase, a Phase II detoxification enzyme, in HepG2 cells.


© 2006 Standard Process Inc. All rights reserved.
Standard Process Inc.
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